論文・総説
Specific N-terminal amino acids potentiate the periplasmic expression of single-chain variable fragments in Escherichia coli
Yoshio Hanyu and Mieko Kato
BioTechniques Vol. 74, No 2, 1-6, 2023
High-yield expression of periplasmic single-chain variable fragments by solid Escherichia coli cultures
Yoshio Hanyu and Mieko Kato
BioTechniques Vol. 72, No 1, 2021
Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins
Yoshio Hanyu and Mieko Kato
Molecules 25(12). 2905-2916, 2020
Potentiating Antigen-Specific Antibody Production with Peptides Obtained from In Silico Screening for High-Affinity against MHC-II
Yoshio Hanyu, Yuto Komeiji and Mieko Kato
Molecules 24(16). 2949-2960, 2019
Single-step colony assay autoinduction of scFv expression for the screening of antibody libraries
Mieko Kato and Yoshio Hanyu
BioTechniques Vol.66, No. 4, 194-197, 2019
Colony Assay for Antibody Library Screening
Mieko Kato and Yoshio Hanyu
Antibody Engineering 103-117, 2018
Single-step colony assay for screening antibody libraries
Mieko Kato and Yoshio Hanyu
Journal of Biotechnology Vol.255, 1-8, 2017
Enzymatic assembly for scFv library construction
Mieko Kato and Yoshio Hanyu
Methods in Molecular Biology Vol.1577, 31-44, 2017
Fusion of Zif268 to the C-terminus of scFvs promotes expression of the active from in the cytoplasm of Esherichia coli
Mieko Kato and Yoshio Hanyu
Biochemistry & Molecular Biology Journal Vol 2, No 1, 1-7, 2016
Screening technologies for recombinant antibody libraries
Mieko Kato and Yoshio Hanyu
Medical Research Archives Vol 2, No 7, 1-14, 2015
Potentiation of antigen-specific antibody production by peptides derived from Ag85B of Mycobacterium tuberculosis
Mieko Kato and Yoshio Hanyu
Journal of Immunological Methods Vol. 417, 45-51, 2014
Construction of an scFv library by enzymatic assembly of VL and VH genes
Mieko Kato and Yoshio Hanyu
Journal of Immunological Methods Vol.396, 15-22, 2013