論文・総説

Combination Screening of a Naive Antibody Library Using E. coli  Display and Single-Step Colony Assay

Yoshio Hanyu and Mieko Kato

BioTechniques  Vol. 72, No 1, 2021

 　

Screening Antibody Libraries with Colony Assay Using scFv-Alkline Phosphatase Fusion Proteins

Yoshio Hanyu and Mieko Kato

Molecules 25(12). 2905-2916, 2020 

 　

Potentiating Antigen-Specific Antibody Production with Peptides Obtained from In Silico Screening for High-Affinity

against MHC-Ⅱ

Yoshio Hanyu, Yuto Komeiji and Mieko Kato

Molecules 24(16). 2949-2960, 2019

 　

Single-step colony assay autoinduction of scFv expression for the screening of antibody libraries 

Mieko Kato and Yoshio Hanyu

BioTechniques Vol. 66, No. 4, 194-197, 2019

 　

Colony Assay for Antibody Library Screening

Mieko Kato and Yoshio Hanyu 

Antibody Engineering  103-117, 2018

 　

Single-step colony assay for screening antibody libraries

 Mieko Kato and Yoshio Hanyu   

Journal of Biotechnology  Vol. 255, 1-8, 2017

 

Enzymatic assembly for scFv library construction

Mieko Kato and Yoshio Hanyu

Methods in Molecular Biology  Vol. 1577, 31-44, 2017

 　

Fusion of Zif268 to the C-terminus of scFvs promotes expression of the active from in the cytoplasm of Esherichia coli

Mieko Kato and Yoshio Hanyu

Biochemistry & Molecular Biology Journal Vol  2, No 1, 1-7, 2016

 　

Screening technologies for recombinant antibody libraries

Mieko Kato and Yoshio Hanyu

Medical Research Archives Vol 2, No 7, 1-14, 2015

 　

Potentiation of antigen-specific antibody production by peptides derived from Ag85B of Mycobacterium tuberculosis

Mieko Kato and Yoshio Hanyu

Journal of Immunological Methods Vol. 417, 45-51, 2014

　 

Construction of an scFv library by enzymatic assembly of VL and VH genes

Mieko Kato and Yoshio Hanyu

Journal of Immunological Methods Vol. 396, 15-22, 2013